Figure 5.

Anti-IL-33 decreases CII-specific proinflammatory cytokine production. Splenocytes (n = 5) were prepared from mice on day 43 and cultured with CII for 96 h. The content of cytokines in supernatants (72 h for IL-6, IL-12, IL-33, sST2, and TNF-α; 96 h for IFN-γ and IL-10) was detected by ELISA. Compared with the control group, the production of IFN-γ (a), IL-6 (b), IL-12 (c), TNF-α (d), and IL-33 (e) in spleen cell cultures of mice with anti-IL-33 treatment was significantly inhibited. There was no significant difference in the content of sST2 in spleen cells between the two groups (f). Data are mean ± SEM of triplicate cultures; n = 5. ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001 by two-way ANOVA with Bonferroni's multiple comparison test.