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. 2020 Dec 22;13(1):15. doi: 10.3390/cancers13010015

Figure 1.

Figure 1

Effects of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) depletion in prostate cancer cells. (A) MALAT1 expression was quantified by qRT-PCR in five different prostate cancer (PC3 n = 6, PC3AR n = 3, DU145 n = 6, LNCaP n = 4, and C27IM n = 5) and one breast cancer (MCF7 n = 4) cell lines at 72 h after transfection with specific (MALAT1), control (LacZ), or no (NT) gapmers. (B) Lactate concentration determined by a colorimetric assay in the cell culture supernatant of PC3, PC3AR, DU145, LNCaP, C27IM, and MCF7 cells after MALAT1 gapmerization as in panel A (n = 3 or 4). Samples were collected after 48 h from transfection. (C,E) mRNA levels of lactate dehydrogenase A (LDHA, n = 3, C) and monocarboxylate transporter 1 and 2 (MCT1 and MCT2, n = 3, D) were quantified by qRT-PCR in PCa cells at 72 h after transfection with MALAT1 or LacZgapmers. (D) Enzymatic activity of lactate dehydrogenase and pyruvate kinase measured in PC3 (n = 3) cells 24 h after transfection with MALAT1 or LacZgapmers. Results are depicted as fold induction vs. LacZgapmer taken as 1 (black straight line). Individual values with mean +/−SEM are shown (red circles for MALAT1 gapmer, black circles for untreated cells, NT). Non-parametric paired two-tailed Student’s t-test determined statistical significance. * p ≤ 0.05 MALAT1gapmer vs. LacZgapmer.