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. 2020 Dec 29;11:610052. doi: 10.3389/fpls.2020.610052

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Copyright © 2020 Feldeverd, Porter, Yuen, Iwai, Carrillo, Smith, Barela, Wong, Wang, Kang, Matsumoto and Christopher.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoblot analysis using the PDI9-specific antiserum of proteins isolated from flowers (Fl), stems (St), Leaves (Lf), siliques (Sq), roots (Rt), and pollen (Pol), of Arabidopsis wild type (WT) and the pdi9-1 single knockout mutant (KO) and the 35S:PDI9 overexpressor (OE) lines (A). The 52 kD PDI9 protein band is denoted. The lower panel under the blot is the Coomassie stained protein gel. (B,C) Subcellular localization of PDI9 using TEM immunolabeling with the PDI9 antiserum (and 15 nm gold anti-rabbit secondary antiserum) on WT roots (B) and pollen (C). Arrowheads point to immunogold particles associated with the ER. Other structures are indicated for reference, V, vacuole; G, Golgi apparatus. Scale bars are 500 nm.