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. 2021 Jan 11;23:21. doi: 10.1186/s13075-020-02411-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Expression of collagen genes were altered following overexpression and knockdown of miR-320a. Normal human pulmonary fibroblasts were transfected with miR-320a mimics, miR-320a inhibitors, and scramble as control. Transfected cells were fixed, permeabilized, blocked, immunostained, and subjected to immunofluorescence. Nuclei were stained with DAPI (blue channel), and COL1A1 was stained with an anti-COL1A1 antibody (green channel). a Images were acquired by confocal microscopy at an original magnification of × 10. Scale bar, 10 μm. b, c Quantitized immunofluorescence intensity of type I collagen in the miR-320a overexpression group was dramatically decreased compared to the control group, whereas the intensity in the miR-320a downregulation group was significantly increased. Experiments were repeated at least 3 times. Values are means ± SD. *p < 0.05 versus scramble controls. ***p < 0.001 versus scramble controls