Fig. 5.

MiR-200a-3p directly target YAP1. A Putative complementary sites within miR-200a-3p andYAP1 predicted by bioinformatics analysis (TargetScan). B Dual luciferase reporter assays demonstrated that YAP1 is a direct target of miR-200a-3p. C-D YAP1 had high expression in BC tissues compared with adjacent normal tissues. E YAP1 had high expression in BC cell lines. F Positive correlations between the expression of hsa_circ_0005273 and YAP1 were found with Pearson’s correlation analysis in BC tissue samples (n = 120). G Negative correlations between the expression of miR-200a-3p and YAP1 were found with Pearson’s correlation analysis in BC tissue samples (n = 120). H The protein levels of YAP1/p-YAP1 to be increased after transfection of miR-200a-3p inhibitor in BC cells by Western blotting. I, K, M and O Si-YAP1 rescued the promotive effects of miR-200a-3p-inhibitor in BC cells by MTT assay and transwell assays. J, L and N Western blotting showed that si-YAP1 could partly rescue the high expression of YAP1, p-YAP1 and PCNA caused by miR-200a-3p-inhibitor in BC cells. *p < 0.05, **p < 0.01,*** p < 0.001,**** p < 0.0001