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. 2021 Jan 8;218(3):e20200839. doi: 10.1084/jem.20200839

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© 2021 Deuse et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 7. — CD47 overexpression using plasmid vector transfection. (A) CD47 overexpression in B2M⁻^/⁻CIITA⁻^/⁻ hiPSCs was achieved by plasmid vector transfection, and out of this B2M⁻^/⁻CIITA⁻^/⁻ CD47 tg (plasmid) hiPSC pool, 28 clones were picked and expanded. Clone 21 showed the highest CD47 expression. (B) Clone 21 iPSCs were differentiated into hiECs, and the CD47 expression level was assessed by flow cytometry (mean ± SD, four independent experiments). (C and D) hiEC clone 21 was challenged with IL-2–activated human primary NK cells (C) or human macrophages (D). Graphs show mean ± SD and three independent replicates per group and time point; three different E:T ratios are shown.