Figure 10.

The inhibitory effects of C16 and Ang-1 on pulmonary vascular permeability and edema in ARDS rats. (A-Q) Assessment of Evans blue (EB) extravasation in LPS-induced ARDS rats (n = 3). EB extravasations assay was performed to observe the pulmonary vascular permeability. EB staining was visualized via red laser excitation at 405nm. Without the EB leakage, the pulmonary tissue appeared light blue due to autofluorescence of the tissue, as shown in the normal control in Q. The tissue color turned red (A, C, E, G, I, J, K, M, O) due to medium or high vascular permeability. However, if there is no or only low vascular permeability, only the small amount of tissue that contacts EB will turn to red (B, D, F. H, L, N, P). The arrow in panel R points to where the EB was leaking from the blood vessel, and the arrow in panel S shows that a great amount of EB (red color) has bled out from a blood vessel. However, at the same time point, the blood vessel in the C16 and Ang-1–treated group did not exhibit clear EB leakage, as indicated by the arrow in panel T. Scale bar = 100 μm.