Figure 7.

The suppressive effects of C16 and Ang-1 on protein expression of inflammation-associated factors in vivo.Western blot assays were performed to examine the expression of inflammation-associated factors and leukocyte infiltration as indicated in lung tissues of ARDS rats. β-actin was used as an internal control. Treatment with C16 and Ang-1 suppressed the expression of the inflammatory cytokine TNF-α (A, B), neutrophil specific marker MPO (at early stage following insult, C,D) and the expression levels of leukocyte marker CD68 (at late stage following insult, E, F) as well as were all significantly increased after LPS insult, whereas C+A treatment obviously inhibited these increases in protein expression.Quantification of Western blot data by normalization to β-actin expression. *P<0.05 vs. normal group, #P<0.05 vs. LPS group (Panel B: The results power calculation of TNF-α expression between normal control and L+C+A group at 1 h post-insult = 0.051; at 4 h post-insult = 0.999. Panel D:The results power calculation of MPO expression between Normal control and L+C+A group at 1 h post-insult = 0.943.Panel F: The results power calculation of CD68 expression between normal control and L+C+A group at 1 h post-insult = 0.408. Power calculation between other groups = 1, group sizes per measurement = 5).