KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rabbit anti-Cdk1 phospho-Tyr15 | Cell Signaling Technology | #9111L; RRID:AB_331460 |
| mouse anti-Cdk1 | Santa Cruz Biotechnology | #SC-54; RRID:AB_627224 |
| mouse anti-tubulin | Santa Cruz Biotechnology | #SC-32293; RRID:AB_628412 |
| rabbit anti-HSP27 phospho-Ser82 | Cell Signaling Technology | #2401; RRID:AB_331644 |
| mouse anti-HSP27 | Cell Signaling Technology | #2402; RRID:AB_331761 |
| rabbit anti-p38 MAPK | Cell Signaling Technology | #9212; RRID:AB_330713 |
| rabbit anti-p38 MAPK phospho-Thr180/Tyr182 | Cell Signaling Technology | #9211; RRID:AB_331641 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| PD0166285 | EMD Millipore | #513028 |
| Cycloheximide | Sigma-Aldrich | 01810-5G |
| SB202474 | EMD Millipore | #559387 |
| SB202190 | Sigma | S7067 |
| SB203580 | EMD Millipore, | #559387 |
| MK-1775 | Selleckchem | S1525 |
| horse serum | GIBCO | #16050-114 |
| fetal bovine serum | Axenia Biologix | #F001 |
| choleratoxin | Sigma-Aldrich | C8052-2mg |
| insulin | Sigma-Aldrich | I1882-100mg |
| hygromycin B | Invitrogen | 10687-010 |
| FuGENE6 | Promega | E2691 |
| Collagen (PureCol) | Advanced BioMatrix | #5005-100ML |
| 96-well glass bottom plate | Cellvis | P96-1.5H-N |
| Experimental Models: Cell Lines | ||
| MCF10A | Laboratory of Tobias Meyer | |
| HEK293T | ATCC | CRL-3216 |
| HeLa | ATCC | CCL-2 |
| hTERT RPE-1 | ATCC | CRL-3216 |
| MCF10A eYFP-PCNA H2B-mTurquoise | This study | N/A |
| MCF10A eYFP-PCNA H2B-mCherry | This study | N/A |
| hTERT RPE-1 eYFP-PCNA H2B-mTurquoise | This study | N/A |
| HeLa eYFP-PCNA H2B-mTurquoise | This study | N/A |
| Recombinant DNA | ||
| pTRIP-EF1α | (Dardalhon et al., 2001) | N/A |
| pTRIP-EF1α-eYFP-PCNA | This study | N/A |
| CSII-EF | (Spencer et al., 2013) | N/A |
| CSII-EF- H2B-mTurquoise | This study | N/A |
| CSII-EF- H2B-mCherry | This study | N/A |
| Software and Algorithms | ||
| Mathematica 10 and 12 | Wolfram Research | https://www.wolfram.com/mathematica/ |
| Graphpad Prism 8.0.2 and 8.4 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Fiji (ImageJ, version 2.00-rc-49/1.51e) | (Schindelin et al., 2012) | https://imagej.net/Fiji |
| MetaXpress 5.1 software | Molecular Devices | https://www.moleculardevices.com/ |
| Excel | MIcrosoft | N/A |
| Python 3.7.3 | (Van Rossum and Drake, 2009) | https://www.python.org/ |
| MATLAB R2015b | MathWorks | https://www.mathworks.com |
| Other | ||
| ImageXpress Micro System Standard Model | Molecular Devices | https://www.moleculardevices.com/ |