Fig. 2.

1H7, but not AF305, inhibits M22 and GO-Ig stimulation of HA secretion. a GOFs were stimulated by maximally effective doses of IGF-1 or the TSAb M22 in the absence (control) or presence of the IGF-1R-blocking antibodies AF305 or 1H7, and HA secretion was measured after 5 days. Both AF305 and 1H7 inhibited HA secretion stimulated by IGF-1 but only 1H7, not AF305, inhibited stimulation by M22. Modified from Krieger et al. [7]. b GOFs were stimulated by purified GO-Igs from 57 patients in the absence (not shown) or presence of the IGF-1R-blocking antibody AF305 or 1H7, and HA secretion was measured after 5 days. The data are presented as percent of control in the absence of AF305 or 1H7. In contrast to AF305, 1H7 decreased HA secretion by all GO-Ig preparations (mean inhibition of 67%) by inhibiting TSHR/IGF-1R crosstalk. ****p< 0.0001 versus GO-Ig control by Student's t test [7].