Fig. 4.

Mutational impact at SDPs across the RBM. (A) Pairwise sequence alignment of SARS-CoV-2 RBM and consensus sequence of Sarbecovirus clade 2. Black triangles indicate amino acid mismatches. SDP positions are depicted as pink letters. (B) Boxplot distributions of ΔΔG values resulting from mutating SDPs and non-SDPs using FoldX (PDB ID: 6LZG). Significant differences were computed using a Wilcoxon unpaired two-sample test (p-value < 0.01). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)