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. 2021 Jan 12;57(2):191–206. doi: 10.1007/s11626-020-00532-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Figure 5. — Brain spheroids for the 3D modeling of the central nervous system. Developed spheroids show a diverse population of both (a) neuronal and (b) glial cells throughout the bulk of the organoid. (c) Myelination is also readily apparent and the progression of myelination within the spheroid can be assessed via both (d) immunohistochemistry and (e) TEM. Reproduced from Pamies et al. (2017), under Creative Commons license.