Figure 3 |. Phylogenetic analysis and metabolic reconstruction of Species A (‘Ca. Manganitrophus noduliformans’).
a, Bayesian phylogram based on 1532 aligned 16S rRNA nucleotide positions. Sequences clustering within the three previously described classes within this phylum are collapsed into separate nodes. Species A clusters with not-yet-cultivated members of the Trogloglia, a distinct class within the bacterial phylum Nitrospirae (Nitrospirota). See Extended Data Fig. 5a–c for greater detail and identifiers. Scale bars = evolutionary distance (0.1 substitutions-per-site average). b, Hypothetical model of e− flow from extracellular Mn(II) to the energy and anabolic systems of Species A. The oxidation is hypothesized to be mediated by several expressed outer membrane complexes (orange), with subsequent e− transfers to periplasmic carriers. The bulk of e− flow is towards generating proton motive force via terminal oxidases (TO, green) during O2 respiration. The remaining e- flow would be to motive force dissipating, reverse electron transport complexes (blue), generating the low-potential e− carriers required for rTCA cycle mediated CO2-fixation. Gene expression values represent the mean of independent cultivation experiments (n=7). See Supplementary Table 4 for identifiers and transcript levels for each gene, and Supplementary Notes 7 and 8 for more detailed explanations of the diagrams.