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. 2021 Jan 7;29(1):82–87.e3. doi: 10.1016/j.str.2020.10.003

Figure 2.

Figure 2

Serial cryoFIB/SEM of Frozen-Hydrated Primary Cells from Control and Patient Fibroblasts

(A) A representative cryoSEM image from a stack of 575 serial micrographs recorded from a control fibroblast cell cultured on an EM grid.

(B–I) An image gallery of subcellular structures and organelles observed in the control cell, including three consecutive slices of a phagosome entering the cell (B–D), two consecutive slices of an endosome (E and F, star), a multivesicular body (E and F, MV), Golgi complexes (G, asterisks), tubular-shaped mitochondria (G and H, arrow), and vacuoles (I and V).

(J) A representative cryoSEM image from a stack of 2018 serial micrographs recorded from a patient fibroblast cell cultured on an EM grid.

(K–O) An image gallery of subcellular structures and organelles observed in the patient cell, showing endoplasmic reticulum (K, ER), nuclear pores (K and L, arrowheads), Golgi complex (M, asterisk), mitochondria (L and N, arrows), and vacuoles (M, O, and V). Arrows, mitochondria; asterisks, Golgi; stars, endosome; PG, phagosome; MV, multivesicular body; V, vacuoles; arrowheads, nuclear pore; orange arrows, platinum GIS coating; green arrow, cell membrane; ER, endoplasmic reticulum. Scale bars, 1 μm.