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. 2021 Jan 8;14:27–39. doi: 10.2147/IJGM.S275016

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© 2021 Yin and Lin.

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Down-regulating miR-21 suppresses malignant aggressiveness of CSCC cells. (A) RT-qPCR of miR-21 expression in HSC-1 and A431 cells transfected with miR-21 inhibitor (*P < 0.05, **P < 0.01 according to the two-way ANOVA); (B) Colony formation assay of cell colony-forming ability (*P < 0.05 according to the two-way ANOVA); (C) EdU assay of cell proliferation (*P < 0.05 according to the two-way ANOVA); (D) Scratch test of cell migration ability (*P < 0.05 according to the two-way ANOVA); (E) Transwell assay of cell invasion ability (*P < 0.05 according to the two-way ANOVA); (F) Flow cytometry of cell cycle distribution (*P < 0.05 according to the two-way ANOVA); (G) Flow cytometry of cell apoptosis (*P < 0.05 according to the two-way ANOVA); The results represent the average of three experiments.