Core needle biopsies were incubated in saline solution for 0 (baseline, 100%), 1, 15, 30, or 60 min of (A) cold ischemia or (B) warm ischemia. (C) Follow-up study of stability of full-length and pY1234/1235-MET in core biopsies during 0, 1, 2, 3, 4, 5, and 8 min of cold ischemia. For all graphs, error bars are mean ± SD, n = 4-6; a single asterisk (*) denotes P < 0.05 from baseline by Student’s t test. (D) Western blot of extracts of core biopsies after 0, 1, 15, 30 or 60 min of cold ischemia probed with the indicated antibodies.