(A) pY1235-MET:MET ratio and (B) pY1356-MET:MET ratio during daily treatment with PF02341066. Tumor samples were analyzed at 4 and 24 h after dose 1, then 4, 12 and 24 h after dose 4 (D4), 12 h after dose 5 (D5), and 4, 12, and 24 h after dose 10 (D10). Mean ± SD, all groups n = 2-6 per dose per time point. The dotted line indicates the LSC in pMET:MET ratio from the vehicle-treated group of 45%; changes larger than this are attributed to drug effect. (C) Absolute levels of full-length MET plotted over time following daily doses of 12.5 and 25 mg/kg PF02341066 or vehicle; data normalized to extracted protein.