Figure 2.

LA induces apoptotic cell death. (A) Lung cancer cells were pre-treated with CQ (20 µM) and U0126 (10 µM) for 1 h, then exposed to LA (20 µM) for 36 h, then flow cytometric analysis was carried out to investigate cell death. (B) Lung cancer cells were pre-treated with z-VAD-fmk (10 µM) for 1 h, then exposed to LA (20 µM) for 36 h, then cell viability was investigated by MTT assay. (C) Lung cancer cells were treated with indicated concentration of LA for 36 h, then the expression of indicated proteins was examined by Western blot. N = 3. *P<0.05, ***P<0.001.