Figure 4.

LA downregulates the expression of RIP1 in a HSP90-independent manner. (A) Lung cancer cells were treated with indicated concentration of LA for 36 h, then the expression of indicated proteins was examined by Western blot. (B) Lung cancer cells were treated with indicated concentration of LA for 36 h, then the expression of indicated proteins was examined by Western blot. (C) Lung cancer cells were exposed to LA (20 µM) for 8 h, then total RNA was extracted, and the mRNA level of indicated proteins was examined by q-RT-PCR, β-Actin was detected as an input control. (D) Lung cancer cells were pre-treated with LA (20 µM) or PBS for 4 h, then exposed to CHX (200 µg/mL) for indicated times, then the expression of indicated proteins was examined by Western blot. GAPDH or tubulin were detected as an input control. # P>0.05.