Figure 6.

ERK-mediated autophagy resists LA-induced anticancer effects. (A) Lung cancer cells were treated with LA (20 µM) for indicated times, then the expression of indicated proteins was examined by Western blot. (B) Lung cancer cells were pre-treated with U0126 (10 µM) for 1 h, then exposed to LA (20 µM) for 12 h, then the expression of indicated proteins was examined by Western blot. (C–E) Lung cancer cells were pre-treated with U0126 (20 µM) for 1 h, then exposed to LA (20 µM) for 36 h, then morphology was examined and cell viability was investigated by MTT assay, and the expression of indicated proteins was examined by Western blot. GAPDH or tubulin were detected as an input control. N = 3. **P<0.0.1.