Table 1. Candidate proteins from the four pathways associated with cold tolerance of winter rapeseed.
| Pathway | Common DAP | KO id | Annotation [EC no.] | Fold (NST1/NFT1) | Regulation | Fold (NST2/NFT2) | Regulation | q-value |
|---|---|---|---|---|---|---|---|---|
| Phenylalanine, tyrosine and tryptophan biosynthesis | XP_013728819.1 | K00766 | anthranilate phosphoribosyltransferase(trpD)[EC:2.4.2.18] | 2.46 | Up | 0.41 | Down | 0.00 |
| XP_013676058.1 | 2.46 | Up | 0.41 | Down | 0.00 | |||
| XP_013722305.1 | 1.74 | Up | 0.47 | Down | 0.00 | |||
| XP_013653143.1 | K00815 | tyrosine aminotransferase(TAT)[EC:2.6.1.5] | 1.87 | Up | 0.47 | Down | 0.00 | |
| XP_013718039.2 | 1.74 | Up | 0.47 | Down | 0.00 | |||
| XP_013657201.1 | K01658 | anthranilate synthase component II(trpG)[EC:4.1.3.27] | 1.62 | Up | — | — | 0.00 | |
| XP_022545953.1 | 1.62 | Up | — | — | 0.00 | |||
| XP_022565834.1 | K01735 | 3-dehydroquinate synthase(aroB)[EC:4.2.3.4] | 1.52 | Up | — | — | 0.00 | |
| XP_013661385.1 | 1.52 | Up | — | — | 0.00 | |||
| XP_013640777.1 | K13832 | 3-dehydroquinate dehydratase(aroDE)[EC:1.1.1.25] | 2.30 | Up | 0.54 | Down | 0.00 | |
| XP_013726187.1 | K14454 | aspartate aminotransferase, cytoplasmic(GOT1)[EC:2.1.6.6] | 0.50 | Down | 2.30 | Up | 0.00 | |
| XP_013721995.1 | 11.31 | Up | — | — | 0.00 | |||
| XP_013663182.1 | 2.30 | Up | 0.47 | Down | 0.00 | |||
| XP_013664180.1 | K15849 | bifunctional aspartate aminotransferase and glutamate(PAT)[EC:2.6.1.78] | 2.00 | Up | — | — | 0.00 | |
| XP_013707917.2 | 2.00 | Up | — | — | 0.00 | |||
| Flavonoid biosynthesis | XP_022571603.1 | K00487 | trans-cinnamate 4-monooxygenase(CYP73A)[EC:1.14.13.11] | 2.00 | Up | 0.38 | Down | 0.00 |
| XP_022557738.1 | 2.00 | Up | 0.38 | Down | 0.00 | |||
| XP_013683255.1 | 2.00 | Up | 0.38 | Down | 0.00 | |||
| XP_013678442.1 | K05278 | flavonol synthase(FLS) [EC:1.14.11.23] | 1.74 | Up | 0.47 | Down | 0.00 | |
| XP_022562120.1 | 1.74 | Up | 0.47 | Down | 0.00 | |||
| XP_013715671.1 | 2.83 | Up | — | — | 0.00 | |||
| XP_013743978.1 | K13065 | shikimate O-hydroxycinnamoyltransferase (HCT) [EC:2.3.1.133] | 2.64 | Up | 0.23 | Down | 0.00 | |
| XP_013692583.1 | 2.64 | Up | 0.23 | Down | 0.00 | |||
| Lysine degradation | XP_013672989.1 | K14085 | aldehyde dehydrogenase family 7 member A1(ALDH7A1)[EC:1.2.1.31] | 0.33 | Down | 3.48 | Up | 0.00 |
| XP_013707249.1 | K00128 | aldehyde dehydrogenase (NAD+)(ALDH)[EC:1.2.1.3] | 2.46 | Up | — | — | 0.00 | |
| XP_013745666.1 | 2.64 | Up | — | — | 0.00 | |||
| XP_022565853.1 | K00626 | acetyl-CoA C-acetyltransferase(atoB)[EC:2.3.1.9] | 0.38 | Down | 1.87 | Up | 0.00 | |
| XP_022550217.1 | K14157 | alpha-aminoadipic semialdehyde synthase(AASS)[EC:1.5.1.8] | 0.38 | Down | 3.25 | Up | 0.00 | |
| Ubiquinone and other terpenoid-quinone biosynthesis | XP_013749046.1 | K01904 | 4-coumarate—CoA ligase(4CL)[EC:6.2.1.12] | 1.62 | Up | — | — | 0.00 |
| XP_013723238.1 | K00457 | 4-hydroxyphenylpyruvate dioxygenase(HPD, hppD)[EC:1.13.11.27] | 0.35 | Down | 3.73 | Up | 0.00 | |
| XP_013695640.1 | 0.35 | Down | 3.73 | Up | 0.00 | |||
| XP_013723237.1 | 0.35 | Down | 3.73 | Up | 0.00 | |||
| XP_013695641.1 | 0.35 | Down | 3.73 | Up | 0.00 | |||
| XP_013719625.1 | K09834 | tocopherol cyclase(VTE1, SXD1)[EC:5.5.1.24] | — | — | 1.62 | Up | 0.00 |
“-” means no significant differential-accumulation.