Highlights	Class of Recommendation	Level of Evidence
•AVS is the key diagnostic step to identify patients to refer for unilateral laparoscopic adrenalectomy.	I	B
•AVS is recommended in all PA patients fulfilling certain requirements.	I	B
•AVS must be performed under well-standardized conditions, i.e. after correction of hypokalemia and switching the interfering medications.	II	B
•AVS can be performed by simultaneous or sequential cannulation, under basal or cosyntropin-stimulated conditions, following pre-defined protocols.	II	B
•The measurement of plasma cortisol, metanephrine or androstenedione concentration, must be used to establish success and calculate the lateralization index.	II	B