Table 3.

Gradient flow profiles of the mobile phase.

Time (min)	Flow (mL/min)	Solvent A (0.1% formic acid in H2O (v/v))	Solvent B (0.1% formic acid in acetonitrile (v/v))
0–2	300	95	5
2–30	300	5	95
30–40	300	95	5