Table 2.
The data of ESI-MS and the binding affinity and stability of peptide/HLA-A∗0201 complex.
| Antigen | Peptide | ESI − MS[M ± H]± | MFI | FIb | DC50c | |
|---|---|---|---|---|---|---|
| Calculated | Observed | |||||
| Cell | - | - | - | 5.64 | - | |
| Background | - | - | - | 131 | - | - |
| Rv0194 | p160 | 936.25 | —a | ND | ND | ND |
| Rv0933 | p186 | 958.08 | 959.90 | 465 | 2.52 | <2 h |
| p208 | 987.16 | 988.57 | 479 | 2.59 | <2 h | |
| Rv1218c | p24 | 916.04 | 916.89 | 569 | 3.32 | >4 h |
| P200 | 989.24 | 989.78 | 366 | 1.75 | >4 h | |
| p158 | 930.02 | 930.85 | 439 | 2.35 | >4 h | |
| p280 | 941.09 | 939.98 | 353 | 1.62 | <2 h | |
| p165 | 987.14 | 987.69 | 479 | 2.6 | > 2 h | |
| Rv1819c | p74 | 926.21 | —a | ND | ND | ND |
| p70 | 944.25 | —a | ND | ND | ND | |
| p122 | 1025.24 | 1026.6 | 177 | 0.35 | ND | |
| Rv2209 | p158 | 872.07 | 873.4 | 462 | 2.54 | >4 h |
| Rv2477c | p182 | 1051.16 | 1051.77 | 379 | 1.87 | >6 h |
| p316 | 984.16 | 984.8 | 348 | 1.58 | >2 h | |
| p362 | 991.24 | 991.85 | 367 | 1.72 | > 2 h | |
| p265 | 1116.28 | 1116.87 | 315 | 1.36 | ND | |
| p173 | 1011.27 | 1011.89 | 272 | 1.15 | ND | |
| Rv1877 | p71 | 920.12 | 919.59 | 419 | 2.17 | >2 h |
| p95 | 932.17 | —a | ND | ND | ND | |
| p215 | 960.22 | —a | ND | ND | ND | |
| p295 | 922.16 | —a | ND | ND | ND | |
| Rv2994 | p223 | 1019.29 | 1020.62 | 430 | 2.28 | <2 h |
| p100 | 1047.3 | —a | ND | ND | ND | |
| p307 | 958.27 | —a | ND | ND | ND | |
| Rv3728 | p264 | 858.11 | —a | ND | ND | ND |
| p313 | 1118.38 | —a | ND | ND | ND | |
| p110 | 932.17 | —a | ND | ND | ND | |
| p54 | 1007.15 | —a | ND | ND | ND | |
| Rv2459c | p469 | 904.07 | 905.42 | 440 | 2.36 | >4 h |
| COX-2d | p321 | 999.6 | 1000.3 | 408 | 2.11 | >4 h |
aPurification failed. bFI = [mean fluorescence intensity (MFI) of the peptide − MFI of the background]/[MFI of the background]. cDC50: estimate of the time required for the loss of 50% of the peptide/HLA-A∗0201 complexes stabilized at time 0 h. dPositive control peptide. ND: not determined. Background group was added with the corresponding solution buffer.