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Effect of LpqH on the secretion of IL-1β and LDH by Ana-1 macrophages. Cells were first primed by LPS and then were grouped into different groups, including the negative control group, or treated with LpqH (0.1 μg/mL, 0.5 μg/mL, and 1.0 μg/mL) for 2 h, and treated with LpqH 1.0 μg/mL and 50 mM KCl or 5 μM ATP for 2 h. ELISA was performed to detect levels of (a) IL-1β and (b) LDH in the culture supernatant. ∗∗P < 0.01 compared with control; #P < 0.01 compared with LpqH 1.0 μg/mL.
