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. 2021 Jan 6;10(1):bio057232. doi: 10.1242/bio.057232

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Ctdp1 KO leads to cell death in MEFs. (A) Ctdp1^+/+ and Ctdp1^flox/flox MEF genotyping at 0, 3, 5, and 7 days post Lenti-Cre transduction. PCR products: floxed allele (1326 bp), wild-type allele (1246 bp), and exons 3–4 deleted (453 bp). (B) Western blot of Ctdp1 in Ctdp1^+/+ and Ctdp1^flox/flox MEFs with and without Lenti-Cre transduction and 3 days of puromycin selection. (C,D) Flow cytometry analysis of Annexin V-APC/PI staining of Ctdp1^+/+ and Ctdp1^flox/flox MEFs before (C) and 5 days after (D) Lenti-Cre transduction. (E) TUNEL staining in Ctdp1^+/+ and Ctdp1^flox/flox MEFs with and without Lenti-Cre transduction. Presented as mean±s.e.m., n=3. P-values ≤0.05 are displayed.