Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 6;10(1):bio056804. doi: 10.1242/bio.056804

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 3. — Transplantation of allogeneic SSCs through the efferent duct. (A) Transplantation process of allogeneic SSCs through the efferent duct. (B) H&E staining of non-transplanted and transplanted testes. Red arrows indicate restored germ cell layers in the seminiferous tubules, and red stars indicate regenerated immature sperm. Scale bars: 50 μm. (C) PLZF immunohistochemistry of testes showing restored spermatogonia with brown positive staining (red arrows) in the seminiferous tubules. The insets represent high magnification of pointed cells in break line boxed areas. Scale bars: 50 μm. (D) Sperm collected from epididymis 2 months post-transplantation and age and breed-matched WT mice. Red boxes point to regenerated sperm with EGFP expression in Etv5^−/− mice and WT sperm which are EGFP negative in the same conditions. Scale bars: 20 μm. (E) Heterologous Etv5 and EGFP DNA were measured to determine the origin of epididymal sperm. NT, non-transplanted testes; 1, transplanted testes; 2, sperm collected from epididymis of SSC-transplanted testes; SSCs, donor cells used for transplantation.