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. 2020 Dec 26;12(24):26047–26062. doi: 10.18632/aging.202253

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Copyright: © 2020 Guo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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The autophagy flux analysis. (A) Represent images were captured under fluorescence microscope with 1000× magnification. Autophagosomes were labelled by both green and red punctate dots and autolysosomes were labelled by red puncta only. (B) Quantification of relative LC3 puncta was plotted. (C, D) The autophagy-related proteins were assessed using Western blot. *P<0.05 compared with the control group treated with PBS; #P<0.05 compared with the H2O2-induced aging group; &P<0.05 compared with the H2O2+CQ group.