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SRD5A3 inhibition suppresses cell proliferation of HCC in vitro. SMMC-7721 and QGY-7703 cell lines were infected with sh-SRD5A3 or sh-NC lentivirus. The knockdown efficiency of SRD5A3 was confirmed by (A–C) Western blot and (D, E) qRT-PCR analyses. (F–H) Colony formation assays were performed and analyzed. (I, J) CCK-8 assays were conducted to measure cell viability (two-way ANOVA analysis). NC, negative control. *, P value < 0.05.
