Fig. 2.

Effects of cell type-specific Nr5a1 disruption. Nr5a1 expression starts at the progenitor cell stage and reaches a plateau in matured fetal Leydig cells (FLCs) and Sertoli cells. (A) Nr5a1 was disrupted in FLCs or progenitor cells using the Cyp11a1-Cre or FLE-Cre mouse line, respectively. Thus, Nr5a1 expression (indicated by broad arrows) was expected to increase once and then be abolished in the FLCs or progenitor cells (broken arrows). (B) Nr5a1 was disrupted in Sertoli cells using the Amh-Cre mouse line. The expression was expected to increase once and then be abolished after the differentiation of Sertoli cells (indicated by a broad arrow and a broken arrow). The effects of Nr5a1 on the presence of the cell types and the cellular functions in the conditional knockout mice are summarized on the right side.