Figure 4.

Levels of human serum albumin and human hepatocyte chimerism

The percentage of human hepatocytes at the time of death was determined by quantifying levels of human and mouse RPP30 by qPCR on total DNA extracted from livers using primer/probe sets against human and mouse RPP30. Values were corrected for the presence of non-hepatocyte mouse cells as described in Materials and Methods. (A and B) Levels of human hepatocyte chimerism are shown for each treatment group (A) and for individual mice (B). (C and D) Levels of human serum albumin were quantified by ELISA and are shown for all animals prior to initial HBV challenge (C) or for surviving animals at day 56 after AAV administration (200 days after HBV challenge) (D). (E) The percentage of human hepatocytes at death for animals surviving 56 days or longer after AAV is also shown. Asterisks indicate mice with detectable HBV gene editing. Error bars indicate SD; p values were generated using one-sided t tests. Values for the six mice that survived for 56 days or longer are color coordinated (colored dots).