Figure 5.

Liver-associated viral DNA levels at the time of death

(A–C) Levels of total HBV DNA (A), cccDNA (B), and total HBV DNA per cccDNA molecule (C) found in human hepatocytes of each liver were assayed by ddPCR using primers that detect total HBV DNA, cccDNA, and hRPP30. Grouped (left panel) and individual mouse (right panel) values are shown. Mouse livers were snap-frozen at necropsy, or as soon as possible following death. Total HBV DNA levels were analyzed using total DNA extracted from livers by a QIAGEN DNeasy blood and tissue kit. cccDNA levels were analyzed using DNA that was extracted from livers using a modified Hirt procedure, and then digested with T5 exonuclease. Error bars indicate SD; p values were generated using one-sided t tests.