Figure 1.
Downregulation of IL-18R during kidney Trm differentiation
(A) Experimental design. 104 congenically marked naive P14 T cells were transferred into B6 recipients followed by LCMV Arm infection on the next day.
(B and C) (B) Fourteen days after infection and (C) 45 days after infection, representative FACS profiles of kidney CD8+ T cells are shown. (i.v., intravascular and e.v., extravascular).
(D) The percentage of IL-18Rlo cells in e.v. CD69+ P14 T cells.
(E) The composition of i.v. (left) versus e.v. (right) P14 T cells at day 45 post-infection.
(F) The percentage of CD103+ cells in kidney P14s versus SI-IEL P14s at day 45. Pooled results from five independent experiments are shown in (D).
Representative results from two to three independent experiments are shown in (B), (C), (E), and (F). Each symbol in (D) and (F) represents the results from an individual mouse. ∗∗, p < 0.01; ∗∗∗, p < 0.001; and ∗∗∗∗, p < 0.0001 by one-way ANOVA with Tukey multi-comparison post-test. See also Figure S1. Bar graphs indicate the mean (±S.D.).