Figure 4.
IL-18Rlo T cells represent a bona fide kidney-resident population
(A) Parabiosis experimental design for (B) to (D). Naive P14 T cells with distinct congenic markers were separately transferred into B6 mice followed by LCMV Arm infection. D30 p.i., pairs of mice carrying congenically different P14s were surgically connected and examined 19–21 days later.
(B) Representative FACS profiles of kidney CD8+ T cells (n = 10).
(C) The composition (host- versus partner-derived) of each indicated P14 subset.
(D) Parabiosis experimental design for (E). D12 p.i., pairs of mice carrying congenically different P14s were surgically connected and examined 15 days later.
(E) Percentage of host-derived versus partner-derived cells within different P14 subsets are shown (n = 8).
Pooled results from two independent experiments are shown in (C) and (E). N.S., not significant, ∗∗, p < 0.01; ∗∗∗, p < 0.001 by paired Student t test or one-way ANOVA with Tukey multi-comparison post-test. Bar graphs indicate the mean (±S.D.).