Figure 5.

TGF-β and type I IFN control the downregulation of IL-18R during kidney Trm differentiation

Congenically marked control and Tgfbr2^−/− P14 T cells were co-transferred into B6 mice followed by LCMV infection. Fourteen days post-infection, representative FACS profiles of pre-gated P14 T cells in both i.v. and e.v. compartments are shown in (A). (B) Day 14 and (C) day 42–45 post-infection, the ratio of (Tgfbr2^−/− P14/Control P14) in each pre-gated kidney P14 subset. (D) Experimental design for (E) to (G). At day 4 and day 7 post-infection, 1 mg anti-IFNAR-1 or isotype control antibody was given i.p. Kidney P14 cells were examined later. (E) Day 30 post-infection, representative FACS profiles of kidney CD8⁺ T cells. (F) Day 12 and (G) day 30 post-infection, the percentage of IL-18R^lo cells in donor P14 (left) or host CD8⁺ T cells (right) in the kidney. Each symbol in (B), (C), (F), and (G) represents the results from an individual mouse. Pooled results from two to five independent experiments are shown in (B), (C), (F), and (G). N.S., not significant, ∗∗, p < 0.01, ∗∗∗; p < 0.001; and ∗∗∗∗, p < 0.0001 by Student t test or one-way ANOVA with Tukey multi-comparison post-test. See also Figures S4–S6. Bar graphs indicate the mean (±S.D.).