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. 2021 Jan 12;11:754. doi: 10.1038/s41598-020-80254-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Imaging experiments lay-out. (a) Reflectance confocal microscope with heating apparatus and thermal camera. External temperature controller (1) and power supply (2) were used to heat and maintain the desired tissue temperature. Thermal camera (3) was positioned above the sample stage (4) to provide independent temperature monitoring. (b) Picture of mouse ear positioning on the sample stage (1) of the confocal microscopy system. Ear tissue was heated using the same heating apparatus (2) as for the integrating sphere measurements.