Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 12;12:330. doi: 10.1038/s41467-020-20123-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Assembly of the ULK1-FIP200-Atg13-Atg101 complex primes the induction of Beclin1-VPS34-VPS15 complex to generate phosphatidylinositol-3-phosphate (PIP3) lipids in membranes of different organelles (e.g., endoplasmic reticulum). The ULK1 and the Beclin1 complexes are negatively or positively modulated by mTOR and AMPK, respectively. The PIP3 signal recruits the conjugation systems (LC3/GABARAP and Atg5/Atg12/Atg16) of autophagy that induces the covalent linking of phospholipids with LC3-GABARAP proteins for membrane’s commitment to becoming autophagosomes. Then the committed membrane (phagophore assembly site or isolation membrane) elongates and closes to form a double-layered vesicle (autophagosome) that is docked to the lysosome (Ly) for cargo destruction. The endoplasmic reticulum site where autophagosomes are formed, which is revealed by DFCP1 protein, is named omegasome.