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. 2021 Jan 12;11:567. doi: 10.1038/s41598-020-80783-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) The Passing Bablok regression line allows visual inspection of agreement between manual and automatic segmentation. The intercept represents constant differences while the regression line slope represents proportional differences among methods, none of which were significant. (b) The observed bias (difference (Y–X)) is 0.61 (SD 2.28) and 94% of the differences are included within the 95% agreement limit (see text for detailed interpretation).