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. 2021 Jan 12;11:720. doi: 10.1038/s41598-020-80606-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Description of the large microplastics (lMP) found in the caddisfly natural cases. (A) The arrow highlights a lMP fibre included in caddisfly natural case. (B) Abundance of lMP found in the natural cases of caddisflies. Bars indicate 1–99 percentile. The three groups are significantly different (p < 0.01, Kruskal Wallis). (C) Black squares on the grid indicate the presence of lMP divided per shape (fragment, fibre, film) in each natural case of caddisflies. The image (A) is obtained by the software PowerPoint (ver. 2002, https://www.microsoft.com/it-it/microsoft-365/powerpoint); (B) by the software GraphPad Prism (ver. 8.4.2, https://www.graphpad.com/support/faq/prism-842-release-notes/), (C) by the software PAST (ver. 3.14, http://folk.uio.no/ohammer/ past/)⁶¹.