Table 2.
Plasmid | Description | Source |
---|---|---|
pET28A NP | T7lac; kanR | SK lab collection |
pCDNA M | T7; ampR | SK lab collection |
pUG35GFP | PMET25 GFP; ampR Sc-URA3 | From Prof. Johannes Hegemann |
pUG34 DsRed-SKL | PMET25 DsRed.SKL; ampR; Sc-HIS3 | Kuravi et al. (2006) |
pUG35 NDVNP GFP | PMET25 NP-GFP; ampR Sc-URA3 | This study |
pUG36GFP | PMET25 GFP; ampR Sc-URA3 | From Prof. Johannes Hegemann |
pUG36GFP NDVNP | PMET25 GFP-NP; ampR Sc-URA3 | This study |
pUG35 NDVM GFP | PMET25 M-GFP; ampR Sc-URA3 | This study |
pHS12-mCherry | PT7 preCOX4-mCHERRY; ampR Sc-LEU2 | Addgene plasmid 25,444 |
pSM1959 | Sec63 mRFP; ampR Sc-LEU2 | Addgene plasmid 41,837 (Metzger et al. 2008) |