Table 1. Predicted B-cell epitopes from SARS-CoV-2 S protein .
| Sequence | Position | Entropy score a | Entropy score b | CBPS* |
| AYTNSF | 27–32 | 0.545 | 0.346 | 0.67 |
| HAIHVSGTNGTKRFDNP* | 66–82 | 0.381 | 0.489 | 0.79 |
| TEKSNI | 95–100 | 0.050 | 0.115 | 0.66 |
| DSK | 111–113 | 0.115 | 0.231 | 0.64 |
| QFCNDPFLGVYYHKNNKSWMESEFRVYSSANNC* | 134–166 | 0.400 | 0.567 | 0.71 |
| VSQ | 171–173 | 0.0 | 0.0 | 0.62 |
| LMDLEGKQGNFKNLR* | 176–190 | 0.320 | 0.370 | 0.78 |
| SKHTPINLVRDLPQGFS* | 205–221 | 0.299 | 0.367 | 0.74 |
| LHRSYLTPGDSSSGWTA* | 244–260 | 0.435 | 0.652 | 0.81 |
| YNEN | 279–282 | 0.075 | 0.173 | 0.62 |
| NATRFASVYAWNRKRI | 343–358 | 0.091 | 0.130 | 0.63 |
| VIRGDEVRQIAPGQTGKIADYNYKLPD | 401–427 | 0.071 | 0.102 | 0.66 |
|
WNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEI YQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPY* |
436–508 | 0.319 | 0.351 | 0.76 |
| CGPKKSTNLVKNKCVNFNFNG | 525–545 | 0.064 | 0.132 | 0.65 |
| TESNKKFLPFQQFGRDIADTTDAVRDPQTLEI* | 553–584 | 0.107 | 0.195 | 0.68 |
| TNTS | 602–605 | 0.0 | 0.0 | 0.62 |
| HVNNSYEC | 655–662 | 0.116 | 0.173 | 0.66 |
| YQTQTNSPRRARSVASQ* | 674–690 | 0.210 | 0.367 | 0.68 |
| SLGAENSVAYSNNSIAIPTN* | 698–717 | 0.080 | 0.138 | 0.69 |
| DSTECS | 745–750 | 0.0 | 0.0 | 0.65 |
| VEQDKNTQ | 772–779 | 0.110 | 0.173 | 0.64 |
| KQIYKTPPIKDFGG | 786–799 | 0.114 | 0.148 | 0.67 |
| LPDPSKPSKRSF* | 806–817 | 0.074 | 0.115 | 0.73 |
| QYGDCLGDIAA | 836–846 | 0.068 | 0.126 | 0.63 |
| QNVLYENQK | 913–921 | 0.0 | 0.0 | 0.64 |
| RLDKVEA | 983–989 | 0.008 | 0.0 | 0.64 |
| GQSKR | 1035–1039 | 0.0 | 0.0 | 0.60 |
|
PAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQR NFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELD* |
1069–1146 | 0.053 | 0.089 | 0.68 |
Abbreviation:CBPS, Consensus B-cell epitope prediction score.
aThe average Shannon entropy score calculated using the multiple sequence alignment of the reference sequence of SARS-CoV-2 S protein (accession no. YP_009724390.1) and the hundred different isolates of spike glycoprotein of CoVs. b The average Shannon entropy score calculated using pairwise sequence alignment of the reference sequence of the S glycoproteins of SARS-CoV (accession no. NP_828851.1) and SARS-CoV-2. Final selected B-cell epitopes are indicated by *.