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. 2021 Jan 12;11:543. doi: 10.1038/s41598-020-80748-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of NLRP3 inflammasome activation in bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs). Both types of cells were treated with LPS (1 μg/ml) and ATP (5 μM), and the changes with or without fucoxanthin (40 μM) pre-treatment 4 h ago were compared. (A,B) Expression levels of the mature form of IL-1β and proteolytically processed caspase-1 in the culture media of BMDMs (A) and BMDCs (B). (C,D) Western blot analysis of NLRP3 inflammasome component expression in LPS/ATP treated BMDMs (C) and BMDCs (D) with or without fucoxanthin. (E,F) ASC oligomerization in cross-linked pellet of BMDMs (E) and BMDCs (F) was also analyzed by immunoblot.