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. 2021 Jan 12;11:788. doi: 10.1038/s41598-020-80876-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro apoptosis detection in cervical cancer HeLa cells. (A) Representative pictures (black arrows indicating morphological changes) of HeLa cells treated with 10 μM and 30 μM CDDP with/without the combination of 10 μM RDM-B (BV10) for 12 h and then incubated with the CellEvent Caspase-3/7 Green Detection Reagent and analyzed using fluorescence microscopy. (B) Percentages of activated caspases 3/7. (C)–(H) Early/late apoptosis measurements of HeLa cells treated with 20 μM and 40 μM CDDP with/without the combination of 20 μM BV10 for 18 h using an Annexin V-FITC Apoptosis Detection Kit, where the cells were double stained with Annexin-V-FITC and PI. Quantitative analyses of the cell images in A and flow cytometry data in (C)–(H) were performed using an ImageJ software (https://imagej.nih.gov/ij/) and a FlowJo software (https://www.flowjo.com/solutions/flowjo), respectively.