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. 2021 Jan 12;11:1643. doi: 10.1038/s41598-020-80763-7

Author Correction: Frequency-dependent drug screening using optogenetic stimulation of human iPSC-derived cardiomyocytes

Hendrik Lapp 1,#, Tobias Bruegmann 1,2,#, Daniela Malan 1, Stephanie Friedrichs 1, Carsten Kilgus 1, Alexandra Heidsieck 3, Philipp Sasse 1,
PMCID: PMC7804085  PMID: 33436963

Correction to: Scientific Reports 10.1038/s41598-017-09760-7, published online 29 August 2017

This Article contains an error in the Experimental Procedures section under subheading ‘Cell culture and generation of ChR2-expressing cardiomyocytes’, where data is omitted in the first paragraph,

“1 × 106 Cor4U cardiomyocytes derived from hiPSC were purchased from Axiogenesis (Cologne, Germany) and cultivated in Cor4U Complete Culture Medium (Axiogenesis) in T25 flasks. This cell line has been previously characterized in detail17,35,36 and consists of ventricular (~60%), atrial (~20%) and pacemaker-like (20%) cardiomyocytes37. For expression of ChR2, Cor4U cardiomyocytes were transduced in the T25 flask with an adeno-associated virus (AAV) at 6.6 × 104 genome copy numbers per cell.

should read:

“1 × 106 Cor4U cardiomyocytes derived from hiPSC were purchased from Axiogenesis (Cologne, Germany) and cultivated in Cor4U Complete Culture Medium (Axiogenesis) in T25 flasks. This cell line has been previously characterized in detail17,35,36 and consists of ventricular (~60%), atrial (~20%) and pacemaker-like (20%) cardiomyocytes37. After studies had been completed short tandem repeat testing by Axiogenesis/NCardia determined that Cor.4U cardiomyocytes were derived from the human embryonic stem cell line RUES2. The different provenience of these well characterized cardiomyocytes does neither change the findings nor the interpretation of the data. Thus the term “iPSC” used in this paper refers to “pluripotent stem cells”. For expression of ChR2, Cor4U cardiomyocytes were transduced in the T25 flask with an adeno-associated virus (AAV) at 6.6 × 104 genome copy numbers per cell.


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