Figure 3.

High doses of UT did not promote cell senescence, but they induced apoptosis in cultured myoblasts. (a) C₂C₁₂ cells were treated with a mixture of 100 µg/mL IS and 100 µg/mL PC (UT) for 24, 48, or 72 h. Senescence-associated β-galactosidase activity was analysed using confocal microscopy using the fluorogenic substrate C₁₂FDG. A representative experiment is shown. The bar graph represents mean ± SEM from ten experiments. (b) C₂C₁₂ cells were treated with a mixture of 100 µg/mL IS and 100 µg/mL PC (UT) for 48 h in the presence or absence of 0.25 mM probenecid (PB). Apoptosis was analysed using the Annexin V test by flow cytometry. A representative experiment is shown. The bar graph represents the mean ± SEM from ten experiments. *p < 0.05 versus CT. ^$p < 0.05 versus UT.