Table 2.
Kinetic data for ME, FumC, and AspA measured by conventional steady-state solution assays.
| Aspartate synthesis (downstream) | Aspartate oxidation (upstream) | |||||
|---|---|---|---|---|---|---|
| ME | FumC | AspA | ME | FumC | AspA | |
| kcat (s−1) | 17 ± 1.1 | 220 ± 54 | 503 ± 33 | 34 ± 3.1 | 220 ± 29 | 359 ± 15 |
| KM (mM) | 8.6 ± 2.2 | 1.5 ± 0.11 | 0.19 ± 0.03 | 0.7 ± 0.22 | 0.6 ± 0.2 | 9 ± 1.6 |
| kcat/Km(M−1 s−1) | 2.0 × 103 | 1.47 × 105 | 2.65 × 106 | 4.86 × 104 | 3.67 × 105 | 4.0 × 105 |
| τ (1/kcat, ms) | 58 | 4.5 | 2 | 29 | 4.5 | 2.7 |
Numbers refer to mean values (N = 3 independent replicates) followed by standard errors.