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. 2021 Jan 12;12:325. doi: 10.1038/s41467-020-20094-3

Fig. 2. Tuning binding site strengths alters inducible promoter behavior.

Fig. 2

a Pcombo library schematic consists of all combinations of one of ten proximal LacI-binding sites, four −10 elements, four −35 elements, and ten distal LacI sites. b Uninduced MPRA expression for all assayed Pcombo variants. Grid positions for the −10 and −35 motifs are arranged according to median induced expression, from the weakest to consensus sites (−10:TATAAT and −35:TTGACA). Gray boxes indicate sequences that were not measured by the assay. c Uninduced expression for assayed Pcombo variants containing consensus σ70-binding sites. d Fold change for all assayed Pcombo variants. Fold change is determined by the ratio of MPRA expression at 1 mM IPTG relative to 0 mM IPTG. e Fold change for all assayed Pcombo variants containing consensus σ70-binding sites. Source data are available in the Source Data file.