Figure 4.

Piezo1 activation probability, ${[{\mathit{Ca}}^{2+}]}_i$ characteristics, and the percentages of cell detached and membrane porated show a shear stress-dependency under PL = 100 ms. (a) The average temporal intracellular calcium ratio profile of P1TF cells in response to insonification at 100 ms PL, 20% DC, 60 s TT but different shear stress levels. n = 6 for each data point, error bars represent $\pm \mathrm{std}$. (b) Intracellular calcium wave speed (C_ICW) in P1TF cells at different shear stress levels. n = 6, *p < 0.05, **p < 0.01, ***p < 0.001, and ‘ns’ denotes not significant. Two-tailed t test. (c) Response duration in P1TF cells at different shear stress levels (n = 6). (d) Responsive rate of P1TF and P1KO cells at various shear stress levels (n ~ 20 for each bar). (e) Peak normalized intracellular calcium ratio change in P1TF and P1KO cells at various shear stress levels (n ~ 6 for each case). Note: the data presented in (a) to (e) were based on cells that were neither detached nor porated following insonification. (f) Percentage of detached and porated cells over all the cells in the insonified region during the experiment at different shear stress levels (total number of cells analyzed at each shear stress level in the experiment: N ~ 80). Two-tailed t test in (b), (c) and (e), Fisher’s exact test in (d) and (f). *p < 0.05, **p < 0.01, ***p < 0.001, and ‘ns’ denotes not significant.