Figure 6.

Piezo1 activation probability, ${[{\mathit{Ca}}^{2+}]}_i$ characteristics, and the percentages of cell detached and membrane porated show a PL dependence at a constant shear stress level ($\mathrm{\tau }$ = 50 dyne/cm²). (a) The average temporal intracellular calcium ratio profile of P1TF cells in response to insonification at $\mathrm{\tau }$ = 50 dyne/cm², DC = 20%, TT = 60 s but different PL. n = 6 for each data point, error bars represent $\pm \mathrm{std}$. (b) Intracellular calcium wave speed (C_ICW) in P1TF cells at different PL (n = 6). (c) Response duration in P1TF cells at various PL (n = 6). (d) Response rate of P1TF and P1KO cells at various PL (n ~ 20 for each bar). (e) Peak normalized intracellular calcium ratio change in P1TF and P1KO cells at various PL (n ~ 6 for each case). Note: the data presented in (a) to (e) were based on cells that were neither detached nor porated following insonification. (f) Percentage of detached and porated cells over all the cells in the experiment at different PL. (total number of cells analyzed under each condition: N ~ 80). Two-tailed t test in (b), (c) and (e), Fisher’s exact test in (d) and (f). *p < 0.05, **p < 0.01, ***p < 0.001, and ‘ns’ denotes not significant.