Figure 6.
Quantification of primary neutrophil and HL-60 swarming. (a) Chemotactic index (CI) and speed over time, towards zymosan particle clusters in (i) primary neutrophils, (ii) DdHL-60, (iii) nDdHL-60. (b) The speed of migration increases with time towards zymosan particle clusters (scale bar: CI above 0.8 indicates the cells that are chemotaxing toward zymosan particle. speed (µm/min) = 4.5 means cell moving toward zymosan particle). (c) Primary neutrophils (blue curve) accumulation on targets is fast and form a bigger swarm and dHL-60 neutrophil (orange curve) aggregation proceeds fast and then continues slower over time. (d) Comparison of area of swarm formed between primary neutrophils (PN) and dHL-60 (HL-60). Comparison were performed by unpaired, two-tailed t-test; *P < 0.05). Error bars represent standard deviations for these measurements. Figure 5 shows representative image of experiment of N = 30 swarm spots.